Yes, you can use both Python and Node.js together on the same website, although it might require some careful planning and architecture considerations. Here are a few scenarios where you might combine these technologies:

1. Microservices Architecture: You can build different components of your website as separate microservices, with each service using the technology that best fits its requirements. For example, you could use Node.js for real-time features like chat or notifications, while using Python for the backend that handles business logic and data processing.

2. API Integration: You might have existing services or APIs written in Python or Node.js that you want to integrate into your website. You can expose these services via APIs and have your frontend (which could be built using either technology) consume these APIs.

3. Hybrid Application: In some cases, you might use Python for the backend and Node.js for specific frontend interactions that require real-time updates. This could be achieved by integrating Node.js-based frontend components into a larger Python-powered web application.

4. DevOps and Automation: Both Python and Node.js have strong capabilities in automation and scripting. You could use Python scripts for server management, data processing, and backend tasks, while using Node.js for managing build processes or other frontend-related automation.

5. Serverless Architectures: You can use serverless platforms like AWS Lambda or Azure Functions, which support multiple programming languages including Python and Node.js. Different functions can be written in different languages and used together as part of a larger application.

6. Reverse Proxies: You might use a reverse proxy like Nginx or Apache to route requests to different backend services based on specific URL patterns. This way, you could have Python and Node.js services running on different ports or servers but accessible through a unified domain.